Results
Title

Melatonin Metabolites Protect Human Retinal Pigment Epithelial Cells from Death Caused by Oxidative Stress.

Authors

Ebru, Uras; Derya, Kilicaslan; Hakan, Kurt Akif; Bilge, Alli; Adem, Doğaner

Abstract

This study was aimed to determine the efficacy of 6-hydroxy melatonin and N-acetyl serotoninin protecting human retinal pigment epithelial cells from death brought about by oxidative-stress. The ARPE-19 cells (one of the retinal pigment epithelial cell lines) were used as the test cell culture. Following the application of 500 μM H2O2, ARPE-19 cell line culture was incubated for 20 h to induce oxidative stress damage. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide) assay was used to evaluate the oxidative injury to ARPE-19 cells caused by H2O2 and the effectiveness of 6-hydroxy melatonin and N-acetyl serotonin application in preventing this damage. The results indicated that 6-hydroxy melatonin and N-acetyl serotonin exhibited a therapeutic effect on cells damaged by exposure to oxidative stress. It was concluded that these melatonin metabolites might be useful in treating many retinal conditions linked to the oxidative stress-induced damage. In addition, this study proposed a new idea for developing chemical approaches to the design and synthesis/semisynthesis of new drugs.

Subjects

CHROMATOPHORES; EPITHELIAL cells; OXIDATIVE stress; CELL death; RHODOPSIN; MELATONIN; MELANOPSIN

Publication

Pharmaceutical Chemistry Journal, 2021, Vol 55, Issue 8, p762

ISSN

0091-150X

Publication type

Academic Journal

DOI

10.1007/s11094-021-02490-1