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- Title
Effect of GABA Receptor Agonists or Antagonists Injected Spinally on the Blood Glucose Level in Mice.
- Authors
Sim, Yun-Beom; Park, Soo-Hyun; Kang, Yu-Jung; Kim, Sung-Su; Kim, Chea-Ha; Kim, Su-Jin; Jung, Jun-Sub; Ryu, Ohk-Hyun; Choi, Moon-Gi; Suh, Hong-Won
- Abstract
The possible roles of gamma-amino butyric acid (GABA) receptors located in the spinal cord for the regulation of the blood glucose level were studied in ICR mice. We found in the present study that intrathecal (i.t.) injection with baclofen (a GABA receptor agonist; 1-10 μg/5 μl) or bicuculline (a GABA receptor antagonist; 1-10 μg/5 μl) caused an elevation of the blood glucose level in a dose-dependent manner. The hyperglycemic effect induced by baclofen was more pronounced than that induced by bicuculline. However, muscimol (a GABA receptor agonist; 1-5 μg/5 μl) or phaclofen (a GABA receptor antagonist; 5-10 μg/5 μl) administered i.t. did not affect the blood glucose level. Baclofen-induced elevation of the blood glucose was dose-dependently attenuated by phaclofen. Furthermore, i.t. pretreatment with pertussis toxin (PTX; 0.05 or 0.1 μg/5 μl) for 6 days dose-dependently reduced the hyperglycemic effect induced by baclofen. Our results suggest that GABA receptors located in the spinal cord play important roles for the elevation of the blood glucose level. Spinally located PTX-sensitive G-proteins appear to be involved in hyperglycemic effect induced by baclofen. Furthermore, inactivation of GABA receptors located in the spinal cord appears to be responsible for tonic up-regulation of the blood glucose level.
- Subjects
BLOOD sugar monitoring; GABA receptors; LABORATORY mice; BACLOFEN; GLUCAGON; BICUCULLINE
- Publication
Neurochemical Research, 2013, Vol 38, Issue 5, p1055
- ISSN
0364-3190
- Publication type
Academic Journal
- DOI
10.1007/s11064-013-1016-1