Calbindin-Immunoreactive Interneurons in the Intermediate Zone and Ventral Horn of the Gray Matter of the SC in White Rats.

Porseva, V.

The aim of the present work was to study the topography and structure of 28-kDa calbindin (CB)-containing interneurons in the ventral horn and intermediate zone of the gray matter at levels T and L of the spinal cord (SC). Experiments were performed in adult female Wistar rats ( n = 4) using immunohistochemical and morphometric methods. Cryostat transverse sections of the SC of thickness 14 μm were studied. The intermediate zone of the SC was found to contain two subpopulations of CB-immunoreactive (IR) interneurons in lamina VII in both segments: sympathetic preganglionic neurons and 'partition' cells. The ventral horn also contained two subpopulations of CB-IR interneurons: Renshaw cells in lamina IX in T and lamina VII in L and large interneurons in lamina V in T and lamina VII in L. The only differences between segments were in the larger numbers of Renshaw cells and partition cells in L. The mean cross-sectional areas of CB-IR Renshaw cells and autonomic preganglionic neurons were greater in T, while large interneurons of the ventral horn and partition cells were larger in L.

CALBINDIN; INTERNEURONS; GRAY matter (Nerve tissue); SPINAL cord; LABORATORY rats; IMMUNOHISTOCHEMISTRY; MORPHOMETRICS

Neuroscience & Behavioral Physiology, 2015, Vol 45, Issue 9, p974

0097-0549

Academic Journal

10.1007/s11055-015-0173-x