Extraction of flavonoids and kinetics of purification by macroporous resins from quinoa.

Xiaoyong, Wu; Yuan, Tang; Yanxia, Sun; Dabing, Xiang; Yan, Wan; Qi, Wu; Gang, Zhao

This paper systematically studied the extraction process of total flavonoids from quinoa and the kinetics of macroporous resin purification. On the basis of single-factor experiments, the optimum extraction and purification process of total flavonoids from quinoa was studied by orthogonal experiments. The results showed that the optimum extraction conditions were as follows: ultrasonic time 25 min, temperature 200 °F, solid-liquid ratio 1:10 (g/mL), reflux time 2 h, and ethanol concentration 90%. Under this condition, the total flavonoids extracted from quinoa was 3.859 mg/g. Fifteen percent NaCl was added to salt out protein from flavonoid solution. The best macroporous resin purification process was as follows: pH 2, flow rate 2 BV/h, 0.3 mg/mL flavonoid solution loading, 8 BV water wash with acetone elution. Under this condition, the adsorption rate of purified flavonoids was 80.89%. The purity can be increased from 7.09% of the initial extract to 28.48%.

MACROPOROUS polymers; ANALYTICAL mechanics; QUINOA; FLAVONOIDS; EXTRACTION (Chemistry); GUMS & resins; ACETONE; PROTEINS

Journal of Nanoparticle Research, 2020, Vol 22, Issue 7, p1

1388-0764

Academic Journal

10.1007/s11051-020-04906-7