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- Title
Surface functionalization of magnetic nanoparticles formed by self-associating hydrophobized oxidized dextrans.
- Authors
Farber, Shimon; Ickowicz, Diana; Melnik, Kristie; Yudovin-Farber, Ira; Recko, Daniel; Rampersaud, Arfaan; Domb, Abraham
- Abstract
Magnetic iron oxide nanoparticles surface covered with oleic acid layer followed by a second layer of hydrophobized oxidized dextran aldehyde were prepared and tested for physico-chemical properties and ligand- and cell-specific binding. It was demonstrated that oleic acid-iron oxide nanoparticles coated with an additional layer of hydrophobized oxidized dextran were dispersible in buffer solutions and possess surface aldehyde active groups available for further binding of ligands or markers via imine or amine bond formation. Hydrophobized dextrans were synthesized by periodate oxidation and conjugation of various alkanamines to oxidized dextran by imination. Physico-chemical properties, as separation using magnetic field, magnetite concentration, and particle diameter, of the prepared magnetic samples are reported. The biotin-binding protein, neutravidin, was coupled to the particle surface by a simple reductive amination procedure. The particles were used for specific cell separation with high specificity.
- Subjects
MAGNETIC nanoparticles; SURFACE chemistry; DEXTRAN; IRON oxide nanoparticles; OLEIC acid; LIGAND binding (Biochemistry); CELL separation
- Publication
Journal of Nanoparticle Research, 2014, Vol 16, Issue 6, p1
- ISSN
1388-0764
- Publication type
Academic Journal
- DOI
10.1007/s11051-014-2425-z