Facile internalization of paclitaxel on titania nanoparticles in human lung carcinoma cells after adsorption of serum proteins.

Seo, Ji; Dembereldorj, Uuriintuya; Park, Jin; Kim, Mira; Kim, Semi; Joo, Sang-Woo

The enhanced damage of human carcinoma cells was achieved using paclitaxel (PTX)-assembled TiO nanoparticles (NPs). The crystalline structures and light absorption properties of the TiO NPs were examined by X-ray diffraction patterns and ultraviolet-visible absorption spectroscopic tools. The fabrication of PTX and serum proteins on TiO NPs via self-assembly was checked by diffuse reflectance infrared Fourier transform spectroscopy and dynamic light scattering measurements. The PTX-coated TiO NPs appeared to be well adsorbed by serum proteins. The PTX-coated TiO NPs were found to be well internalized in cancer cells as indicated by transmission electron microscopy. The intracellular aggregation of PTX-coated TiO NPs was assumed to occur after endocytosis leading to an entrapment of NPs in an endosomal or a lysosomal structure. Human lung carcinoma A549 cancer cells were chosen to examine their viability after the cellular uptake of the PTX-coated TiO NPs. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide cell viability assay test indicated that the cell viability should be affected by the concentration of TiO NPs. After applying PTX-coated TiO NPs (50 ppm (μg/mL)) incubating for 24 h, we observed a 30 % further decrease of cell viability for PTX alone in the low concentration range of 0.1-10 nM. Graphical Abstract: [Figure not available: see fulltext.]

TITANIUM dioxide; NANOPARTICLES; LUNG cancer; CANCER cells; BLOOD protein absorption & adsorption; CELL death; LIGHT absorption; MOLECULAR self-assembly

Journal of Nanoparticle Research, 2012, Vol 14, Issue 10, p1

1388-0764

Academic Journal

10.1007/s11051-012-1164-2