Title: Mesoporous silica-coated superparamagnetic particles prepared by pseudomorphic transformation and their application in purification of plasmid DNA.
Authors: Yan, Feng; Sun, Lin; Li, Fubiao; Zhuang, Jiaqi; Wang, Hongliang; Yang, Wensheng
Abstract: Mesoporous silica-coated superparamagnetic particles were prepared via pseudomorphic transformation of pre-made amorphous silica-coated FeO-polymer composite particles using n-cetyltrimethylammonium bromide (CTAB) and 1,3,5-trimethyl benzene (MES) as template. The mesoporous particles presented almost the same size, shape, and magnetic property as the original amorphous particles but an ordered mesoporous shell with wormhole-like pore structure. The pore size of the shells increased from 2.4 to 3.1 and 4.2 nm as the molar ratio of MES/CTAB increased from 0 to 1.0 and 1.5. DNA extraction experiments showed the mesoporous particles were qualified for purification of plasmid DNA from bacterial lysate. Graphical abstract: [Figure not available: see fulltext.]
Subjects: MESOPOROUS materials; SILICA; SURFACE coatings; PARAMAGNETISM; PHASE transitions; CHEMICAL purification; DNA; PLASMIDS; NANOMEDICINE; BROMIDES
Publication: Journal of Nanoparticle Research, 2011, Vol 13, Issue 12, p6613
ISSN: 1388-0764
Publication type: Academic Journal
DOI: 10.1007/s11051-011-0569-7

Mesoporous silica-coated superparamagnetic particles prepared by pseudomorphic transformation and their application in purification of plasmid DNA.

Yan, Feng; Sun, Lin; Li, Fubiao; Zhuang, Jiaqi; Wang, Hongliang; Yang, Wensheng