Herein, carbon nanodots (CNDs) were facilely synthesized using grass as a carbon source by the reflux method. The as-synthesized CNDs were well characterized by UV–Vis spectroscopy, Fluorescence spectroscopy, XRD, FTIR, and SEM. The characterization results revealed that grass-derived CNDs showed strong absorption and fluorescence emission peak and are amorphous with the surface rich in carboxyl and hydroxyl groups. The average particle size is 20 nm with a narrow size distribution. The CNDs showed excellent fluorescent sensitivity and selectivity for the detection of Cu2 ions with a limit of detection as low as 0.89 μM with a linear detection range of 1–20 μM offering a novel sensing platform for Cu2 ion.