Results
Title

Sperm cryopreservation and DNA methylation: possible implications for ART success and the health of offspring.

Authors

Khosravizadeh, Zahra; Khodamoradi, Kajal; Rashidi, Zahra; Jahromi, Malihe; Shiri, Elham; Salehi, Ensieh; Talebi, Ali

Abstract

Despite the beneficial effects of sperm cryopreservation, increased reactive oxygen species (ROS) production during this process can affect spermatozoon structure and function. Moreover, ROS production is associated with elevated DNA damage and alterations in DNA methylation. There is little information about the effects of cryopreservation on epigenetic modulation in sperm and the health of children born with frozen spermatozoa. Considering the potential consequences of cryopreservation in ART-conceived children, it is necessary to assure that cryopreservation does not modify sperm DNA methylation status. This review summarizes reports on epigenetic modifications of spermatozoa during cryopreservation and the probable effects of this process on offspring health. Contradictory results have reported the influence of sperm cryopreservation on DNA methylation in imprinted genes. Multiclinical studies with larger sample sizes under the same conditions of cryopreservation and DNA methylation analysis are needed to make any definitive conclusion about the effect of the cryopreservation process on sperm DNA methylation.

Subjects

DNA methylation; FROZEN semen; CENTRIFUGATION; SPERMATOZOA; REACTIVE oxygen species; DNA analysis

Publication

Journal of Assisted Reproduction & Genetics, 2022, Vol 39, Issue 8, p1815

ISSN

1058-0468

Publication type

Academic Journal

DOI

10.1007/s10815-022-02545-6