Results
Title

Genome instability in AZFc region on Y chromosome in leukocytes of fertile and infertile individuals following exposure to gamma radiation.

Authors

Moghbeli-Nejad, Sahar; Mozdarani, Hossein; Behmanesh, Mehrdad; Rezaiean, Zahra; Fallahi, Parvin

Abstract

Purpose: Men are exposed to various doses of ionizing radiation due to living in regions with high natural background radiation, accidentally, occupationally or for cancer treatment. To study genomic instability of AZFc region to gamma radiation, blood samples from normal, oligozoospermia, and azoospermia individuals were irradiated by a Co-60 source. Methods: Irradiated cells were kept for 48 h in order to repair initial DNA damages. Real time PCR was performed for three markers (SY 1206, SY 1197, SY 579) for testing copy number variation before and after irradiation. Copy number variations were compared by calculation of cycle threshold comparative method. Results: Copy number variations of studied markers in AZFc region (microdeletion and duplication) in all samples after exposure to radiation increased with a dose dependent fashion. The frequency of instability was significantly higher in samples from infertile men in comparison with fertile ones ( p 0.05). This observation might be a possible explanation for induction of azoospermia and oligozoospermia after radiotherapy. Increased frequency of induced microdeletion and duplication in infertile men compared with normal might be attributed to the deficiency in repair systems and the genetic factors involved in incomplete spermatogenesis of infertile men.

Subjects

CANCER treatment; Y chromosome; LEUKOCYTES; GAMMA rays; PHYSIOLOGICAL effects of ionizing radiation; MALE infertility; POLYMERASE chain reaction

Publication

Journal of Assisted Reproduction & Genetics, 2012, Vol 29, Issue 1, p53

ISSN

1058-0468

Publication type

Academic Journal

DOI

10.1007/s10815-011-9626-5