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- Title
Subcritical water extraction of fucoidan from <italic>Saccharina japonica</italic>: optimization, characterization and biological studies.
- Authors
Saravana, Periaswamy Sivagnanam; Tilahun, Adane; Gerenew, Collin; Tri, Vo Dinh; Kim, Nan Hee; Kim, Gun-Do; Woo, Hee-Chul; Chun, Byung-Soo
- Abstract
Subcritical water extraction (SWE) of fucoidan from <italic>Saccharina japonica</italic> was investigated. To optimize, a Box-Behnken design (BBD) was used to study the influences of temperature [100-180 °C], pressure [20-80 bar], solid to liquid (S/L) ratio [0.04-0.09 g mL−1], agitation speed (AS) [100-300 rpm], and reaction time (RT) [5-15 min]. By unraveling the regression equations, the optimal conditions were 127.01 °C, 80 bar, S/L ratio of 0.04 g mL−1, AS of 300 rpm, and RT of 11.98 min. Under these conditions, the crude fucoidan (CF) yield was 13.56%. FT-IR, monosaccharides, TGA and UV-Vis confirmed that the extracted polysaccharides had features of fucoidan. CF showed good antioxidant activities, while anti-proliferative activity showed a moderate cell proliferation for few cell lines. In conclusion, a high yield of CF with good functional activity obtained from SWE might be the favored method for industrial scale production of fucoidan.
- Publication
Journal of Applied Phycology, 2018, Vol 30, Issue 1, p579
- ISSN
0921-8971
- Publication type
Academic Journal
- DOI
10.1007/s10811-017-1245-9