Subcritical water extraction of fucoidan from <italic>Saccharina japonica</italic>: optimization, characterization and biological studies.

Saravana, Periaswamy Sivagnanam; Tilahun, Adane; Gerenew, Collin; Tri, Vo Dinh; Kim, Nan Hee; Kim, Gun-Do; Woo, Hee-Chul; Chun, Byung-Soo

Subcritical water extraction (SWE) of fucoidan from &lt;italic&gt;Saccharina japonica&lt;/italic&gt; was investigated. To optimize, a Box-Behnken design (BBD) was used to study the influences of temperature [100-180 °C], pressure [20-80 bar], solid to liquid (S/L) ratio [0.04-0.09 g mL−1], agitation speed (AS) [100-300 rpm], and reaction time (RT) [5-15 min]. By unraveling the regression equations, the optimal conditions were 127.01 °C, 80 bar, S/L ratio of 0.04 g mL−1, AS of 300 rpm, and RT of 11.98 min. Under these conditions, the crude fucoidan (CF) yield was 13.56%. FT-IR, monosaccharides, TGA and UV-Vis confirmed that the extracted polysaccharides had features of fucoidan. CF showed good antioxidant activities, while anti-proliferative activity showed a moderate cell proliferation for few cell lines. In conclusion, a high yield of CF with good functional activity obtained from SWE might be the favored method for industrial scale production of fucoidan.

Journal of Applied Phycology, 2018, Vol 30, Issue 1, p579

0921-8971

Academic Journal

10.1007/s10811-017-1245-9