Nonlinear biotic ligand model for assessing alleviation effects of Ca, Mg, and K on Cd toxicity to soybean roots.

Chen, Bo-Ching; Wang, Pin-Jie; Ho, Pei-Chi; Juang, Kai-Wei

Developing a nonlinear biotic ligand model (BLM) that considers the geometrical constraints for binding of different cations on biotic ligands will provide more reliable details about the hypothetical mechanism governing the alleviation of cadmium (Cd) toxicity by coexistent cations. Soybean seedlings under Cd stress produced by various activities of coexistent cations such as calcium (Ca), magnesium (Mg), and potassium (K) were hydroponically assayed for Cd toxicity to soybean roots. The Cd activity resulting in 50% reduction of root elongation ( RE), EA , was used for assessing the Cd toxicity to the soybean seedling. Increasing Ca, Mg, and K activities resulted in a significant alleviation of Cd toxicity to soybean roots. This alleviation was markedly higher with increasing Ca and K levels than with increasing Mg level. In addition, EA increased in nonlinear positive relationships with Ca and Mg. The real data obtained from the soybean assay were thus used to develop the nonlinear BLM for Cd rhizotoxicity. Two parameters, competition equivalent and stability constant, indicated the profiles of the geometrical constraint and affinity of Ca, Mg, and K binding on the soybean root surface to alleviate Cd toxicity. Compared with the traditional linear BLM, the nonlinear BLM provided more precise predictions of relative root elongation ( RRE) and EA . Therefore, adopting the nonlinear BLM approach will successfully improve the monitoring and assessment of heavy metal toxicity to terrestrial plants.

CADMIUM analysis; HEAVY metal toxicology; METAL toxicology; TERRESTRIAL heat flow; GROUP 12 elements

Ecotoxicology, 2017, Vol 26, Issue 7, p942

0963-9292

Academic Journal

10.1007/s10646-017-1823-2