Granulocyte colony-stimulating factor enhances bone marrow mononuclear cell homing to the liver in a mouse model of acute hepatic injury.

Shi-Zhu Jin; Xiang-Wei Meng; Xun Sun; Ming-Zi Han; Bing-Rong Liu; Xin-Hong Wang; Li-Ying Sun; Qi Huang; Rui-Bo Zhao; Xiang Ban; Hong-Ying Yu; Hong-Wei Yu; Jin, Shi-Zhu; Meng, Xiang-Wei; Sun, Xun; Han, Ming-Zi; Liu, Bing-Rong; Wang, Xin-Hong; Sun, Li-Ying; Huang, Qi

Background: Experiments have reported that granulocyte colony stimulating factor (G-CSF) can mobilize stem cells. However, few studies have examined the effect of G-CSF on bone marrow mononuclear cell (BMMC) mobilization, in particular regarding their capability to home to acutely injured liver.Aims: The aim of this study was to evaluate the effort of G-CSF on BMMC homing to the liver following chemically-induced hepatic failure.Methods: BMMC were isolated from mice, pre-labeled with PKH26 and infused into the mice in which hepatic injury had been induced followed by administration of G-CSF or vehicle. Livers were studied by fluorescent microscopy after transplantation of pre-labeled BMMC.Results: PKH26 labeled cells were found in liver tissue at 102 ± 10 cells/high power field in the BMMC G-CSF group and 30 ± 5 cells/high power field in the BMMC group, but none in the G-CSF group and the control group (P Conclusion: Transplanted BMMC may home to injured liver, which appears to be enhanced by G-CSF administration.

GRANULOCYTE-colony stimulating factor; STEM cells; BONE marrow; FLUORESCENCE microscopy; LIVER failure; LABORATORY mice; THERAPEUTICS; ANIMAL experimentation; ANIMALS; BIOLOGICAL models; BIOTHERAPY; BIOPSY; BONE marrow transplantation; CELLS; CELL motility; FLOW cytometry; HEMATOPOIETIC stem cells; HYDROCARBONS; IMMUNOHISTOCHEMISTRY; LIVER; MICE; ORGANIC compounds; ALBUMINS; ACUTE diseases; FLUORESCENT dyes

Digestive Diseases & Sciences, 2010, Vol 55, Issue 10, p2805

0163-2116

Academic Journal

10.1007/s10620-009-1117-5