The discrepancy between genetic polymorphism of p53 codon 72 and the expression of p53 protein in Helicobacter pylori-associated gastric cancer in Korea.

Nayoung Kim; Sung-Il Cho; Hye Seung Lee; Ji Hyun Park; Jee Hyun Kim; Joo Sung Kim; Hyun Chae Jung; In Sung Song; Kim, Nayoung; Cho, Sung-Il; Lee, Hye Seung; Park, Ji Hyun; Kim, Jee Hyun; Kim, Joo Sung; Jung, Hyun Chae; Song, In Sung

The p53 gene has been referred to as 'the guardian of the genome' because it controls apoptosis and cell cycle arrest. The purpose of this study was to evaluate the association of p53 codon 72 genetic polymorphism and the p53 immunohistochemistry with Helicobacter pylori-associated gastroduodenal diseases, including gastric cancer. This study included 1,852 subjects: controls and patients with gastric cancer, dysplasia, benign gastric ulcers, and duodenal ulcers (DU). Biallelic polymorphism was genotyped by restriction fragment length polymorphism. Immunohistochemical analysis for the detection of mutant type p53 expression was performed. The frequency of the Pro/Pro allele of the p53 codon 72 was higher in the patients with H. pylori-positive dysplasia than in controls (OR: 2.3, 95% CI: 1.3-4.3), but it was less frequent among patients with a H. pylori-positive DU (OR: 0.5, 95% CI: 0.3-0.8). However, there was no significant association with gastric cancer, including the location, stage, or histological type of gastric cancer. Expression of a mutant type of p53 protein was detected in 6.3% of dysplastic tissues and 26.5% of cancerous tissues compared 0% in the controls. Positive expression was higher in the intestinal type of cancer (34.9%) than in the diffuse type (15.0%; P = 0.001). These results suggest that genetic polymorphism of p53 codon 72 played a role in the determination of H. pylori-associated gastroduodenal diseases, but p53 immunostaining did not correlate with those of the p53 genetic polymorphism analysis.

KOREA; GENETIC polymorphisms; HELICOBACTER pylori infections; GASTRIC mucosa; P53 antioncogene; APOPTOSIS prevention; GASTRODUODENOSTOMY; CANCER; COMPARATIVE studies; DUODENAL ulcers; GENES; HELICOBACTER diseases; HELICOBACTER pylori; IMMUNOHISTOCHEMISTRY; RESEARCH methodology; MEDICAL cooperation; GENETIC mutation; ONCOGENES; PEPTIC ulcer; PROTEINS; RESEARCH; STOMACH tumors; EVALUATION research; GENOTYPES; DISEASE complications

Digestive Diseases & Sciences, 2010, Vol 55, Issue 1, p101

0163-2116

Academic Journal

10.1007/s10620-008-0688-x