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- Title
Molecular cloning and expression analysis of four turmeric MAP kinase genes in response to abiotic stresses and phytohormones.
- Authors
Nanda, S.; Nayak, S.; Joshi, R.
- Abstract
Plant mitogen activated protein kinase (MAPK) cascades comprise a complex network playing a major role in regulating extracellular stimuli as well as developmental processes. The present study involves cloning four MAPKs ( ClMPK1, 3, 4 and 5) from Curcuma longa. All four ClMPKs have fully canonical motifs of MAPK and each is represented by a single copy in the turmeric genome. The analysis of exon-intron junctions revealed conserved nature of ClMPKs across different plant groups. The RT-qPCR analysis showed their expression in mature plant tissues. The transcript analysis using the RT-qPCR shows that the four ClMPKs were differentially regulated by cold, salinity, and drought stresses. ClMPK4 showed a significant upregulation in the presence of NaCl, polyethylene glycol, and mannitol. The time-course expression analysis revealed a marked accumulation of ClMPK1 and ClMPK4 transcripts after mechanical wounding or applications of abscisic acid, HO, methyl jasmonate, and salicylic acid. ClMPK5 showed a unique and pronounced expression in response to hexavalent chromium (Cr).
- Subjects
GENE expression in plants; MOLECULAR cloning; MITOGEN-activated protein kinases; EFFECT of stress on plants; PLANT hormones
- Publication
Biologia Plantarum, 2014, Vol 58, Issue 3, p479
- ISSN
0006-3134
- Publication type
Academic Journal
- DOI
10.1007/s10535-014-0429-2