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- Title
Involvement of G6PDH in heat stress tolerance in the calli from Przewalskia tangutica and Nicotiana tabacum.
- Authors
Gong, H.; Chen, G.; Li, F.; Wang, X.; Hu, Y.; Bi, Y.
- Abstract
Glucose-6-phosphate dehydrogenase (G6PDH) has been implicated in supplying reduced nicotine amide cofactors for biochemical reactions and in modulating the redox state of cells. In this study, the role of G6PDH in thermotolerance of the calli from Przewalskia tangutica and tobacco ( Nicotiana tabacum L.) was investigated. Results showed that Przewalskia tangutica callus was more sensitive to heat stress than tobacco callus. The activity of G6PDH and antioxidant enzymes (ascorbate peroxidase, catalase, peroxidase and superoxide dismutase) in calli from Przewalskia tangutica and tobacco increased after 40 °C treatment, although two calli exhibited a difference in the degree and timing of response to heat stress. When G6PDH was partially inhibited by glucosamine pretreatment, the antioxidant enzyme activities and thermotolerance in both calli significantly decreased. Simultaneously, the heat-induced HO content and the plasma membrane NADPH oxidase activity were also reduced. Application of HO increased the activity of G6PDH and antioxidant enzymes in both calli. Diphenylene iodonium, a NADPH oxidase inhibitor, counteracted heatinduced HO accumulation and reduced the heat-induced activity of G6PDH and antioxidant enzymes. Moreover, exogenous HO was effective in restoring the activity of G6PDH and antioxidant enzymes after glucosamine pretreatment. Western blot analysis showed that G6PDH gene expression in both calli was also stimulated by heat and HO, and blocked by DPI and glucosamine under heat stress. Taken together, under heat stress G6PDH promoted HO accumulation via NADPH oxidase and the elevated HO was involved in regulating the activity of antioxidant enzymes, which in turn facilitate to maintain the steady-state HO level and protect plants from the oxidative damage.
- Subjects
ENZYMES; ANTIOXIDANTS; GLUCOSE 6-phosphatase; DEHYDROGENASES; NICOTINE; TOBACCO
- Publication
Biologia Plantarum, 2012, Vol 56, Issue 3, p422
- ISSN
0006-3134
- Publication type
Academic Journal
- DOI
10.1007/s10535-012-0072-8