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- Title
Somatic embryogenesis in Araucaria angustifolia.
- Authors
A. Wendt dos Santos; N. Steiner; M. Guerra; K. Zoglauer; B. Moerschbacher
- Abstract
Abstract Immature and mature zygotic embryos were used as source of explants for induction of somatic embryogenesis in Araucaria angustifolia. Embryogenic cultures (EC) were only obtained from immature zygotic embryos. Basic medium, carbon source, and genotype showed a significant influence on the formation of stage I somatic embryos (SE). When EC were submitted to maturation conditions, SE continued their individual development until stage II, but mature embryos were not obtained. Proteins secreted by embryogenic cultures were, to a certain degree, genotype specific and included an extracellular class IV chitinase and β-1-3-glucanase.
- Subjects
ARAUCARIACEAE; PLANT tissue culture; BRAZILIAN pine; SOMATIC embryogenesis
- Publication
Biologia Plantarum, 2008, Vol 52, Issue 1, p195
- ISSN
0006-3134
- Publication type
Academic Journal
- DOI
10.1007/s10535-008-0044-1