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Title

Secondary metabolites content and essential oil composition of in vitro cultures of Zingiber montanum (Koenig) Link ex A. Dietr.

Authors

Rajkumari, Sanatombi; Sanatombi, K.

Abstract

Objectives: To determine the secondary metabolite content, antioxidant and phenylalanine ammonia-lyase (PAL) activity as well as essential oil composition of in vitro cultures and field grown rhizomes of Zingiber montanum. Results: Methyl jasmonate-treated cell cultures showed the highest total phenolic content and steroid content of 22.23 mg gallic acid equivalent/g dry weight (DW) and 41.67 mg/g DW, respectively. Callus cultures exhibited the highest tannin content (39.53 mg tannic acid equivalent/g DW) and strongest antioxidant activity (91.05% inhibition of 2,2-Diphenyl-1-picrylhydrazyl or DPPH). The highest saponin (81.76 mg/g DW) and alkaloid (113.97 mg/g DW) contents were obtained in in vitro microrhizomes induced on Murashige and Skoog (MS) medium supplemented with 6% sucrose and 5 mg/l 6-Benzylaminopurine (BAP), and MS medium supplemented with 7% sucrose, respectively. The essential oil content varied in cell cultures and microrhizomes and mainly consisted of fatty acid esters, which are precursors of many secondary metabolites. Trace amounts of terpinen-4-ol (0.21 and 0.27 mg/g) and zerumbone (0.0107 mg/g) were also detected in the in vitro microrhizomes. Conclusion: The results obtained indicate the potential of in vitro cultures of Z. montanum for the production of secondary metabolites.

Subjects

METABOLITES; ZINGIBER; SAPONINS; FATTY acid esters; TANNINS; ESSENTIAL oils; GALLIC acid

Publication

Biotechnology Letters, 2020, Vol 42, Issue 7, p1237

ISSN

0141-5492

Publication type

Academic Journal

DOI

10.1007/s10529-020-02872-7

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