Title: High-content screening of <italic>Aspergillus niger</italic> with both increased production and high secretion rate of glucose oxidase.
Authors: Zhu, Xudong; Sun, Jingchun; Chu, Ju
Abstract: Objectives: To develop a rapid, dual-parameter, plate-based screening process to improve production and secretion rate of glucose oxidase simultaneously in <italic>Aspergillus niger</italic>.Results: A morphology engineering based on CaCO3 was implemented, where the yield of GOD by <italic>A. niger</italic> was increased by up to 50%. Analysis of extracellular GOD activity was achieved in 96-well plates. There was a close negative correlation between the total GOD activity and its residual glucose of the fermentation broth. Based on this, a rapid, plate-based, qualitative analysis method of the total GOD activity was developed. Compared with the conventional analysis method using <italic>o</italic>-dianisidine, a correlation coefficient of −0.92 by statistical analysis was obtained.Conclusion: Using this dual-parameter screening method, we acquired a strain with GOD activity of 3126 U l−1, which was 146% higher than the original strain. Its secretion rate of GOD was 83, 32% higher than the original strain.
Subjects: ASPERGILLUS niger; GLUCOSE oxidase; CALCIUM carbonate; SECRETION; MUTAGENESIS
Publication: Biotechnology Letters, 2018, Vol 40, Issue 1, p103
ISSN: 0141-5492
Publication type: Academic Journal
DOI: 10.1007/s10529-017-2442-y

High-content screening of <italic>Aspergillus niger</italic> with both increased production and high secretion rate of glucose oxidase.

Zhu, Xudong; Sun, Jingchun; Chu, Ju