Design of a multi-enzyme reaction on an electrode surface for an l-glutamate biofuel anode.

Sakamoto, Hiroaki; Komatsu, Tomohiro; Yamasaki, Koji; Satomura, Takenori; Suye, Shin-ichiro

Objectives: To design and construct a novel bio-anode electrode based on the oxidation of glutamic acid to produce 2-oxoglutarate, generating two electrons from NADH. Results: Efficient enzyme reaction and electron transfer were observed owing to immobilization of the two enzymes using a mixed self-assembled monolayer. The ratio of the immobilized enzymes was an important factor affecting the efficiency of the system; thus, we quantified the amounts of immobilized enzyme using a quartz crystal microbalance to further evaluate the electrochemical reaction. The electrochemical reaction proceeded efficiently when approximately equimolar amounts of the enzyme were on the electrode. The largest oxidation peak current increase (171 nA) was observed under these conditions. Conclusion: Efficient multi-enzyme reaction on the electrode surface has been achieved which is applicable for biofuel cell application.

MULTIENZYME complexes; GLUTAMIC acid; BIOMASS energy; NADH dehydrogenase; CHARGE exchange

Biotechnology Letters, 2017, Vol 39, Issue 2, p235

0141-5492

Academic Journal

10.1007/s10529-016-2237-6