Title: Purification and characterization of a novel cis-epoxysuccinate hydrolase from Klebsiella sp. that produces l( )-tartaric acid.
Authors: Cheng, Yongqing; Wang, Li; Pan, Haifeng; Bao, Wenna; Sun, Weirong; Xie, Zhipeng; Zhang, Jianguo; Zhao, Yuhua
Abstract: A strain of Klebsiella sp. BK-58 that produces cis-epoxysuccinate was screened and identified. This novel enzyme hydrolyzes cis-epoxysuccinate to l(+)-tartaric acid and was purified to homogeneity. Its molecular mass was 30.1 kDa determined by MALDI-TOF-MS analysis. It was stable up to 50 °C and from pH 5 to 11 with optima at 50 °C and pH 8.5. The enzyme was metal-independent and was strongly inhibited by 1 mM Cu and Ag, and 1 % (w/v) SDS. The K, V and turnover number ( k) values for cis-epoxysuccinate were 19.3, 2.24 mM min and 220 s, respectively.
Subjects: HYDROLASES; KLEBSIELLA; TARTARIC acid; MOLECULAR weights; ENZYMES
Publication: Biotechnology Letters, 2014, Vol 36, Issue 11, p2325
ISSN: 0141-5492
Publication type: Academic Journal
DOI: 10.1007/s10529-014-1614-2

Purification and characterization of a novel cis-epoxysuccinate hydrolase from Klebsiella sp. that produces l( )-tartaric acid.

Cheng, Yongqing; Wang, Li; Pan, Haifeng; Bao, Wenna; Sun, Weirong; Xie, Zhipeng; Zhang, Jianguo; Zhao, Yuhua