Detection of single nucleotide polymorphisms by PCR conformation-difference gel electrophoresis.

Zhu, Weifeng; Deng, Yan; Jie, Kemin; Luo, Daya; Liu, Zhuoqi; Yu, Lehan; Zeng, Erming; Wan, Fusheng

The most common genetic variations in the human genome, single nucleotide polymorphisms (SNPs), are ideal biomarkers and are used extensively in disease research. Here we introduce a novel method of PCR-conformation-difference gel electrophoresis (PCR-CDGE) used for detecting SNPs. The principle of PCR-CDGE relies PCR products from different homozygous DNA samples showing dissimilar migration patterns upon PAGE due to their conformational differences. PCR products from heterozygous DNA samples may exhibit two or more bands in PAGE because of the existence of DNA homoduplexes and heteroduplexes. In this study, analysis of two SNPs showed that PCR-CDGE is an accurate, simple, rapid, low-cost, and high-throughput genotyping method that could be used in most laboratories.

SINGLE nucleotide polymorphisms; HUMAN genome; GEL electrophoresis; DNA; BIOMARKERS

Biotechnology Letters, 2013, Vol 35, Issue 4, p515

0141-5492

Academic Journal

10.1007/s10529-012-1115-0