We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Detection of single nucleotide polymorphisms by PCR conformation-difference gel electrophoresis.
- Authors
Zhu, Weifeng; Deng, Yan; Jie, Kemin; Luo, Daya; Liu, Zhuoqi; Yu, Lehan; Zeng, Erming; Wan, Fusheng
- Abstract
The most common genetic variations in the human genome, single nucleotide polymorphisms (SNPs), are ideal biomarkers and are used extensively in disease research. Here we introduce a novel method of PCR-conformation-difference gel electrophoresis (PCR-CDGE) used for detecting SNPs. The principle of PCR-CDGE relies PCR products from different homozygous DNA samples showing dissimilar migration patterns upon PAGE due to their conformational differences. PCR products from heterozygous DNA samples may exhibit two or more bands in PAGE because of the existence of DNA homoduplexes and heteroduplexes. In this study, analysis of two SNPs showed that PCR-CDGE is an accurate, simple, rapid, low-cost, and high-throughput genotyping method that could be used in most laboratories.
- Subjects
SINGLE nucleotide polymorphisms; HUMAN genome; GEL electrophoresis; DNA; BIOMARKERS
- Publication
Biotechnology Letters, 2013, Vol 35, Issue 4, p515
- ISSN
0141-5492
- Publication type
Academic Journal
- DOI
10.1007/s10529-012-1115-0