Structural determination and chemical esterification of the sophorolipids produced by Candida bombicola grown on glucose and α-linolenic acid.

Gupta, Reetika; Prabhune, Asmita

The extracellular surface-active glycolipids produced by the yeast, Candida bombicola when grown on glucose and α-linolenic acid, were analyzed by HPLC with electro-spray ionization (ESI−MS) and collision-induced dissociation mass spectrometry. The analysis confirmed that the sophorolipid (SL) mixture contained three different forms of C18:3 SL molecules: free acid, lactone and a diacetylated lactone, which has not been reported previously. Also a minor amount of diacetylated lactone form of C18:1 SL was detected. Further, the SL mixture was subjected to chemical esterification reaction with sodium methoxide. The reaction product was analyzed with ESI−MS and confirmed to be the single homogenous esterified product containing C18:3 moieties in its fatty acid chain.

GLYCOLIPIDS; ESTERIFICATION; YEAST; LINOLENIC acids; LACTONES; COLLISION induced dissociation; HIGH performance liquid chromatography; ELECTROSPRAY ionization mass spectrometry

Biotechnology Letters, 2012, Vol 34, Issue 4, p701

0141-5492

Academic Journal

10.1007/s10529-011-0818-y