Colony-Forming Ability and Residual Foci of DNA Repair Proteins in Human Lung Fibroblasts Irradiated with Subpicosecond Beams of Accelerated Electrons.

Babayan, N. S.; Guryev, D. V.; Vorobyeva, N. Yu.; Grigoryan, B. A.; Tadevosyan, G. L.; Apresyan, L. S.; Chigasova, A. K.; Yashkina, E. I.; Rodneva, S. M.; Tsishnatti, A. A.; Fedotov, Yu. A.; Sarkisyan, N. K.; Manukyan, A. T.; Aroutiounian, R. M.; Osipov, A. N.

We performed a comparative study of the colony-forming ability and the number of residual foci of DNA repair proteins in cultured human lung fibroblasts (MRC-5 cell line) after exposure to subpicosecond beams of accelerated electrons with an energy of 3.6 MeV and quasi-continuous radiation (accelerated electrons with an energy of 4 MeV and X-rays). The yield of damages causing reproductive cell death after pulsed subpicosecond radiation exposure was higher by ~1.8 times than after quasi-continuous radiation exposure. The quantitative yield of residual γH2AX foci (phosphorylated H2AX histone, a protein marker of DNA double breaks) in cells irradiated with subpicosecond beams of accelerated electrons was shown to be ~2.0- 2.5-fold higher than in cells irradiated with quasi-continuous beams of accelerated electrons.

ELECTRON beams; DNA repair; GERM cells; RADIATION exposure; LUNGS; FIBROBLASTS

Bulletin of Experimental Biology & Medicine, 2021, Vol 172, Issue 1, p22

0007-4888

Academic Journal

10.1007/s10517-021-05323-z