Analysis of Cytokines and ATP in Plucked Hair Follicles.

Mikhalchik, E. V.; Morozova, O. V.; Tsimbalenko, T. V.; Kharaeva, Z. F.; Balabushevich, N. G.; Lipatova, V. A.; Gadzhigoroeva, A. G.

The concentrations of ATP, IL-6, and IL-10 were measured in extracts of plucked hair follicles from healthy volunteers (normal values) and patients with androgenetic alopecia and then, ATP, IL-6, and IL-10 content was calculated for each follicle. The resulting values were directly proportional to hair follicle length, except for IL-6. The concentration of extracted ATP correlated with lactate dehydrogenase activity indicating cell damage. In patients with androgenetic alopecia, IL-10 content exceeded the normal values in follicles with a length 2 mm long. The content of IL-6 and IL-10 measured by ELISA was comparable with results of mRNA expression assayed by RT-PCR, which attested to moderate level of gene expression. The content of ATP and IL- 10, but not IL-6 depended on the length of plucked hair follicle and on pathogenetic factors affecting hair growth.

CYTOKINES; LACTATE dehydrogenase; INTERLEUKIN-6; HAIR follicles; HAIR growth; GENE expression

Bulletin of Experimental Biology & Medicine, 2021, Vol 170, Issue 3, p299

0007-4888

Academic Journal

10.1007/s10517-021-05055-0