We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Analysis of Cytokines and ATP in Plucked Hair Follicles.
- Authors
Mikhalchik, E V; Morozova, O V; Tsimbalenko, T V; Kharaeva, Z F; Balabushevich, N G; Lipatova, V A; Gadzhigoroeva, A G
- Abstract
The concentrations of ATP, IL-6, and IL-10 were measured in extracts of plucked hair follicles from healthy volunteers (normal values) and patients with androgenetic alopecia and then, ATP, IL-6, and IL-10 content was calculated for each follicle. The resulting values were directly proportional to hair follicle length, except for IL-6. The concentration of extracted ATP correlated with lactate dehydrogenase activity indicating cell damage. In patients with androgenetic alopecia, IL-10 content exceeded the normal values in follicles with a length 2 mm long. The content of IL-6 and IL-10 measured by ELISA was comparable with results of mRNA expression assayed by RT-PCR, which attested to moderate level of gene expression. The content of ATP and IL- 10, but not IL-6 depended on the length of plucked hair follicle and on pathogenetic factors affecting hair growth.
- Publication
Bulletin of experimental biology and medicine, 2021, Vol 170, Issue 3, p299
- ISSN
1573-8221
- Publication type
Journal Article
- DOI
10.1007/s10517-021-05055-0