Experimental Study of the Effect of Biological Matrixes with Stabilized and Non-Stabilized Platelets on Reparative Process in the Wound Equivalent to Deep Burn.

Borovkova, N. V.; Makarov, M. S.; Ponomarev, I. N.; Andreev, Yu. V.; Storozheva, M. V.; Budaev, A. A.

We studied reparative effect of platelet-filled biological matrixes in the treatment of mice with wounds equivalent to deep burn. The wound coatings were based on decellularized dermal matrix without platelets (control), with native platelets, and with platelets stabilized with 2.5 μM nanosilver. In 3 days, the epithelial layer and derma were absent in all groups and extensive scab was formed. Dermal matrix with platelets simulated intensive migration of macrophages and fibroblasts to the wound bottom; in the control group, this migration was absent. In 14 days, granulation tissue appeared in the wound bottom in animals of all groups; in the experimental groups, the number of vessels was 2-4-fold higher than in the control, though the number of inflammatory cells in experimental groups remained high. On day 21, the scab on the most of the wound area was absent in all animals of the experimental groups and epithelialization and hair growth were pronounced, comparing to control. Nevertheless, in experiment dermal layer was not already completed, inflammation reaction remained.

MATRIX effect; BLOOD platelets; TISSUE wounds; BENTHIC animals; GRANULATION tissue; WOUND healing; HAIR growth

Bulletin of Experimental Biology & Medicine, 2020, Vol 170, Issue 1, p128

0007-4888

Academic Journal

10.1007/s10517-020-05019-w