Methylobacterium pseudosasae sp. nov., a pink-pigmented, facultatively methylotrophic bacterium isolated from the bamboo phyllosphere.

Madhaiyan, Munusamy; Poonguzhali, Selvaraj

A pink-pigmented, Gram negative, aerobic, facultatively methylotrophic bacterium, strain BL44, was isolated from bamboo leaves and identified as a member of the genus Methylobacterium. Phylogenetic analysis based on 16S rRNA gene sequences showed similarity values of 98.7-97.0 % with closely related type strains and showed highest similarity to Methylobacterium zatmanii DSM 5688 (98.7 %) and Methylobacterium thiocyanatum DSM 11490 (98.7 %). Methylotrophic metabolism in this strain was confirmed by PCR amplification and sequencing of the mxaF gene coding for the α-subunit of methanol dehydrogenase. Strain BL44 produced three known quorum sensing signal molecules with similar retention time to C, C and C-HSLs when characterized by GC-MS. The fatty acid profiles contained major amounts of C ω7c, iso-3OH C and summed feature 3 (C ω7c and/or iso-C 2-OH), which supported the grouping of the isolate in the genus Methylobacterium. The DNA G+C content was 66.9 mol%. DNA relatedness of the strain BL44 to its most closely related strains ranged from 12-43.3 %. On the basis of the phenotypic, phylogenetic and DNA-DNA hybridization data, strain BL44 is assigned to a novel species of the genus Methylobacterium for which the name Methylobacterium pseudosasae sp. nov. is proposed (type strain BL44 = NBRC 105205 = ICMP 17622).

METHYLOBACTERIUM; METHYLOTROPHIC bacteria; REPRODUCTIVE isolation; ISOLATION of biotechnological microorganisms; RIBOSOMAL RNA; METHANOL dehydrogenase

Antonie van Leeuwenhoek, 2014, Vol 105, Issue 2, p367

0003-6072

Academic Journal

10.1007/s10482-013-0085-0