Cohnella plantaginis sp. nov., a novel nitrogen-fixing species isolated from plantain rhizosphere soil.

Wang, Li-Ying; Chen, San-Feng; Wang, Lei; Zhou, Yu-Guang; Liu, Hong-Can

A novel bacterium capable of fixing nitrogen was isolated from plantain rhizosphere soil in China. The isolate, designated YN-83, is Gram-positive, aerobic, motile and rod-shaped (0.4-0.6 μm × 1.9-2.6 μm). Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain YN-83 was a member of the genus Cohnella. High similarity of 16S rRNA gene sequence was found between YN-83 and Cohnella ginsengisoli DSM18997 (97.99%), whereas the similarity was below 96.0% between YN-83 and the other Cohnella species. DNA-DNA relatedness between strain YN-83 and C. ginsengisoli DSM18997 was 27.4 ± 6.2%. The DNA G+C content of strain YN-83 was 59.3 mol%. The predominant isoprenoid quinone was MK-7 and the major fatty acids were anteiso-C (44.3%), iso-C (11.3%), iso-C (18.6%) and C (7.7%). The polar lipids of strain YN-83 consist of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, lyso- phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. On the basis of phenotypic and chemotaxonomic properties, 16S rRNA gene sequence, G+C content and DNA-DNA hybridization, strain YN-83 represents a novel species of the genus Cohnella, for which the name Cohnella plantaginis sp. nov. (type strain YN-83 = DSM 25424 = CGMCC 1.12047) is proposed.

CHINA; NITROGEN-fixing bacteria; RHIZOSPHERE; NUCLEIC acid hybridization; PLANT phylogeny; RIBOSOMAL RNA; QUINONE

Antonie van Leeuwenhoek, 2012, Vol 102, Issue 1, p83

0003-6072

Academic Journal

10.1007/s10482-012-9715-1