We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Chemical Tools for Temporally and Spatially Resolved Mass Spectrometry-Based Proteomics.
- Authors
Yuet, Kai; Tirrell, David
- Abstract
Accurate measurements of the abundances, synthesis rates and degradation rates of cellular proteins are critical for understanding how cells and organisms respond to changes in their environments. Over the past two decades, there has been increasing interest in the use of mass spectrometry for proteomic analysis. In many systems, however, protein diversity as well as cell and tissue heterogeneity limit the usefulness of mass spectrometry-based proteomics. As a result, researchers have had difficulty in systematically identifying proteins expressed within specified time intervals, or low abundance proteins expressed in specific tissues or in a few cells in complex microbial systems. In this review, we present recently-developed tools and strategies that probe these two subsets of the proteome: proteins synthesized during well-defined time intervals- temporally resolved proteomics-and proteins expressed in predetermined cell types, cells or cellular compartments- spatially resolved proteomics-with a focus on chemical and biological mass spectrometry-based methodologies.
- Subjects
MASS spectrometry; PROTEOMICS; ACCURACY; CONFIDENCE intervals; BIOMOLECULES; SPECTRUM analysis; PRESERVATION of organs, tissues, etc.
- Publication
Annals of Biomedical Engineering, 2014, Vol 42, Issue 2, p299
- ISSN
0090-6964
- Publication type
Academic Journal
- DOI
10.1007/s10439-013-0878-3