We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Multimeric vitronectin in ascites is involved in fibroblast spreading of EPS in patients on CAPD therapy.
- Authors
Masunaga, Yoshinori; Asakura, Shinji; Muto, Shigeaki; Ando, Yasuhiro; Homma, Sumiko; Kusano, Eiji; Matsuda, Michio; Asano, Yasushi
- Abstract
Background. Although encapsulating peritoneal sclerosis (EPS) is a serious complication of continuous ambulatory peritoneal dialysis (CAPD) therapy, the mechanism of the fibroneogenesis in EPS remains unknown. Because fibroblast adhesion and spreading to the extracellular matrix is the first step in peritoneal fibrosis, we investigated fibroblast spreading factor in ascites obtained from patients with EPS (EPS ascites). Methods. To analyze fibroblast spreading activity, various concentrations of EPS ascites obtained from two EPS patients were coated on culture plates, and then the number of human fibroblasts (TIG-3) that had spread was counted. Each fraction of gel-filtered EPS ascites was also analyzed by this activity. Next, we examined the effect of the addition of Arg-Gly-Asp (RGD) peptides, several antibodies against adhesion molecules, and heparin on the fibroblast spreading activity in the EPS ascites. Results. The fibroblast spreading activity of EPS ascites was about four times greater than that in ascites from a patient with nephrotic syndrome. Two major peaks (peak I and II) of spread cells were obtained when ascites were gel-filtered. The fibroblast spreading activities of the two peaks were abolished by the addition of RGD peptides and polyclonal antibody against vitronectin (VN). Immunoblotting analysis revealed that the two peaks contained VN and that peak I contained multimeric VN. Heparin, at 10 μg/ml, augmented the fibroblast spreading activity of peak I to about three times greater than the control. Conclusions. The results indicate that multimeric VN in EPS ascites plays a potential role in peritoneal fibrogenesis in EPS and that heparin may participate in peritoneal fibrosis in EPS.
- Subjects
VITRONECTIN; CELL adhesion molecules; FIBROBLASTS; CONTINUOUS ambulatory peritoneal dialysis; OUTPATIENT medical care; PERITONEAL dialysis
- Publication
Clinical & Experimental Nephrology, 2003, Vol 7, Issue 2, p150
- ISSN
1342-1751
- Publication type
Academic Journal
- DOI
10.1007/s10157-003-0223-5