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- Title
Irradiation with blue light-emitting diode enhances osteogenic differentiation of stem cells from the apical papilla.
- Authors
Yang, Yaoyao; Zhu, Tingting; Wu, Yan; Shu, Chunxia; Chen, Qiang; Yang, Juan; Luo, Xiang; Wang, Yao
- Abstract
This study aimed to evaluate the effects of low-energy blue LED irradiation on the osteogenic differentiation of stem cells from the apical papilla (SCAPs). SCAPs were derived from human tooth root tips and were irradiated with 0 (control group), 1 J/cm2, 2 J/cm2, 3 J/cm2, or 4 J/cm2 blue light in osteogenic induction medium. Cell proliferation was analyzed using the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay. Osteogenic differentiation activity was evaluated by monitoring alkaline phosphatase (ALP), alizarin red staining, and real-time polymerase chain reaction (RT-PCR). The results of the MTT assay indicated that SCAPs in the LED groups exhibited a lower proliferation rate than those in the control group, and there were statistically differences between the 2 J/cm2, 3 J/cm2, and 4 J/cm2 groups and the control group (P < 0.05). The results of the ALP and alizarin red analyses showed that blue LED promoted osteogenic differentiation of the SCAPs. And 4 J/cm2 blue light upregulates the expression levels of the osteogenic/dentinogenic genes ALP, dentin sialophosphoprotein (DSPP), dentin matrix protein-1 (DMP-1), and osteocalcin (OCN) in SCAPs. Our results confirmed that low-energy blue LED at 1 J/cm2, 2 J/cm2, 3 J/cm2, and 4 J/cm2 could inhibit the proliferation of SCAPs and promotes osteogenic differentiation of SCAPs. Further in vitro studies are required to explore the mechanisms of the effects by low-energy blue LED.
- Subjects
LIGHT emitting diodes; IRRADIATION; STEM cells; CELL proliferation; REVERSE transcriptase polymerase chain reaction; MESENCHYMAL stem cells; CALCIUM metabolism; PROTEIN metabolism; CELL differentiation; TEETH; ALKALINE phosphatase; PROTEINS; BONE growth; CELL culture; OSTEOCALCIN; CELL physiology; FETAL development; GLYCOPROTEINS; PHOSPHOPROTEINS; GENES; RESEARCH funding; PHYSIOLOGICAL effects of radiation
- Publication
Lasers in Medical Science, 2020, Vol 35, Issue 9, p1981
- ISSN
0268-8921
- Publication type
Academic Journal
- DOI
10.1007/s10103-020-02995-3