Investigation of arginine A-specific cysteine proteinase gene expression profiling in clinical Porphyromonas gingivalis isolates against photokilling action of the photo-activated disinfection.

Pourhajibagher, Maryam; Ghorbanzadeh, Roghayeh; Bahador, Abbas

Porphyromonas gingivalis is a significant root canal pathogen capable of causing endodontic infections, which during their treatment may receive sub-lethal doses of photo-activated disinfection (sPAD). As sPAD can influence microbial virulence, this study was designed to evaluate the effect of sPAD on gene expression level of arginine A-specific cysteine proteinase (rgpA), as one of the underlying virulence factors involved in the development of endodontic infection via P. gingivalis strains. To find out the sPAD against 16 clinical isolates of PAD-resistant P. gingivalis that were isolated in vivo, we used toluidine blue O (TBO), methylene blue (MB), and indocyanine green (ICG) as the photosensitizers, which were excited with specific wavelength of light in vitro. Quantitative real-time PCR (qRT-PCR) was then applied to monitor gene expression of rgpA in P. gingivalis isolates to characterize its virulence agent and understand the effect of sPAD on its pathogenicity. Maximal sPAD that could not decrease the count of P. gingivalis isolates were 6.25, 15.6, and 25 μg/mL at fluencies of 171.87, 15.6, and 93.75 J/cm2 for TBO, ICG, and MB, respectively. ICG-sPAD could suppress the rgpA gene expression about 14-fold, while MB and TBO-mediated sPAD could cause the attenuation of rgpA expression about 4.9- and 11.6-fold, respectively. ICG-sPAD with the maximum ability to reduce rgpA gene expression compared with other photosensitizers can be an appropriate candidate for the treatment of endodontic infections.

CYSTEINE proteinases; GENE expression profiling; ARGININE; PORPHYROMONAS gingivalis infections; ROOT canal treatment; TOLUIDINE blue; THERAPEUTICS; RNA metabolism; ARGININE metabolism; CYSTEINE metabolism; ANTIPSYCHOTIC agents; BACTERIAL antigens; GENES; DOSE-response relationship (Radiation); METHYLENE blue; PHENOTHIAZINE; PHOTOSENSITIZERS; POLYMERASE chain reaction; PROTEOLYTIC enzymes; RESEARCH funding; RNA; STERILIZATION (Disinfection); INDOLE compounds; GRAM-negative anaerobic bacteria; PHARMACODYNAMICS

Lasers in Medical Science, 2018, Vol 33, Issue 2, p337

0268-8921

Academic Journal

10.1007/s10103-017-2386-4