A comparative study of red and blue light-emitting diodes and low-level laser in regeneration of the transected sciatic nerve after an end to end neurorrhaphy in rabbits.

Takhtfooladi, Mohammad; Sharifi, Davood; Takhtfooladi, Mohammad Ashrafzadeh

This study aimed at evaluating the effects of red and blue light-emitting diodes (LED) and low-level laser (LLL) on the regeneration of the transected sciatic nerve after an end-to-end neurorrhaphy in rabbits. Forty healthy mature male New Zealand rabbits were randomly assigned into four experimental groups: control, LLL (680 nm), red LED (650 nm), and blue LED (450 nm). All animals underwent the right sciatic nerve neurotmesis injury under general anesthesia and end-to-end anastomosis. The phototherapy was initiated on the first postoperative day and lasted for 14 consecutive days at the same time of the day. On the 30th day post-surgery, the animals whose sciatic nerves were harvested for histopathological analysis were euthanized. The nerves were analyzed and quantified the following findings: Schwann cells, large myelinic axons, and neurons. In the LLL group, as compared to other groups, an increase in the number of all analyzed aspects was observed with significance level (P < 0.05). This finding suggests that postoperative LLL irradiation was able to accelerate and potentialize the peripheral nerve regeneration process in rabbits within 14 days of irradiation.

COMPARATIVE studies; LIGHT emitting diodes; MEDICAL lasers; REGENERATION (Biology); SCIATIC nerve; NEUROPATHY; LABORATORY rabbits; SCIATIC nerve surgery; ANIMAL experimentation; COLOR; ELECTRONICS; LIGHT; RESEARCH methodology; MEDICAL cooperation; NERVOUS system regeneration; NEUROSURGERY; POSTOPERATIVE period; RABBITS; RESEARCH; EVALUATION research; PHYSIOLOGICAL effects of radiation; PHYSIOLOGY

Lasers in Medical Science, 2015, Vol 30, Issue 9, p2319

0268-8921

Academic Journal

10.1007/s10103-015-1813-7