This study is a life cycle assessment (LCA) of conventional asphalt binder versus bio-modified binder that is produced by mixing asphalt binder with bio-binder obtained from swine manure. Both processes were evaluated and compared in terms of their contribution to global warming, using a global warming potential index and energy consumption. This LCA study uses a cradle-to-gate approach for the binder and includes a comparison between the environmental impacts of swine manure in lagoons and the production of bio-binder. The results show the energy consumption and global warming potential improvements after using bio-binder as a sustainable additive.