Results
Title

Pepper vein yellows virus 9: a novel polerovirus isolated from chili pepper in Indonesia.

Authors

Koeda, Sota; Homma, Kanami; Kamitani, Mari; Nagano, Atsushi J.; Taniguchi, Marina; Pohan, Nadya; Kesumawati, Elly

Abstract

In 2017, a leaf sample from a single chili pepper (Capsicum annuum) plant exhibiting yellowing was collected from Aceh province, Indonesia. Total RNA was extracted from this sample, and RNA-Seq analysis was conducted. Putative infecting viruses were detected by mapping the obtained reads to the full-length viral genome sequences available in the GenBank database (7457 sequences) and the de novo-assembled contigs. RNA-Seq analysis detected polerovirus, begomovirus, and amalgavirus sequences, and the polerovirus-like sequences showed strong similarity to those of previously reported pepper vein yellows viruses (PeVYVs). The complete viral genome sequence obtained by RT-PCR had a length of 6023 nt, had the typical genome organization of a polerovirus and showed a high degree of sequence similarity to PeVYV-2 from Israel. Moreover, the predicted amino acid sequence of the P0 protein of the Indonesian isolate was 85.1% to 88.8% identical to those of other PeVYVs. In accordance with the polerovirus species demarcation criteria, this isolate should be assigned to a new polerovirus species, and we propose the name "pepper vein yellows virus 9" (PeVYV-9) for this virus.

Subjects

INDONESIA; ACEH (Indonesia); ISRAEL; PEPPERS; PHYTOPLASMAS; CAPSICUM annuum; HOT peppers; AMINO acid sequence; VIRAL genomes

Publication

Archives of Virology, 2020, Vol 165, Issue 12, p3017

ISSN

0304-8608

Publication type

Academic Journal

DOI

10.1007/s00705-020-04838-6