Title: Establishment and application of an indirect ELISA for porcine circovirus 3.
Authors: Deng, Junhua; Li, Xiangdong; Zheng, DingDing; Wang, Yanwei; Chen, Lingyan; Song, Huanhuan; Wang, Tongyan; Huang, Yuxin; Pang, Wenqiang; Tian, Kegong
Abstract: Porcine circovirus type 3 (PCV3) was initially reported in 2016 in the United States of America. Since then, the virus has been detected on swine farms in Poland, South Korea, and China using PCR. However, a serological survey of PCV3 in pig populations has never been conducted. In this study, for the first time, we established an indirect enzyme-linked immunosorbent (ELISA) assay and performed a national retrospective serological survey for PCV3. Our results showed that the PCV3-postive rate increased from 22.35% to 51.88% between 2015 and 2017. The above results suggest PCV3 has spread widely in China with increased positive rates since 2015.
Subjects: CIRCOVIRUS diseases; ENZYME-linked immunosorbent assay; SEROLOGY; POLYMERASE chain reaction; DIAGNOSIS; SWINE
Publication: Archives of Virology, 2018, Vol 163, Issue 2, p479
ISSN: 0304-8608
Publication type: Academic Journal
DOI: 10.1007/s00705-017-3607-7

Establishment and application of an indirect ELISA for porcine circovirus 3.

Deng, Junhua; Li, Xiangdong; Zheng, DingDing; Wang, Yanwei; Chen, Lingyan; Song, Huanhuan; Wang, Tongyan; Huang, Yuxin; Pang, Wenqiang; Tian, Kegong