Results
Title

Mediatorless amperometric bienzyme glucose biosensor based on horseradish peroxidase and glucose oxidase cross-linked to multiwall carbon nanotubes.

Authors

Xu, Shuxia; Qi, Honglan; Zhou, Shiyi; Zhang, Xinfeng; Zhang, Chengxiao

Abstract

We report on a bienzyme-channeling sensor for sensing glucose without the aid of mediator. It was fabricated by cross-linking horseradish peroxidase (HRP) and glucose oxidase (GOx) on a glassy carbon electrode modified with multiwalled carbon nanotubes (MWNTs). The bienzyme was cross-linked with the MWNTs by glutaraldehyde and bovine serum albumin. The MWNTs were employed to accelerate the electron transfer between immobilized HRP and electrode. Glucose was sensed by amperometric reduction of enzymatically generated HO at an applied voltage of −50 mV (vs. Ag/AgCl). Factors influencing the preparation and performance of the bienzyme electrode were investigated in detail. The biosensor exhibited a fast and linear response to glucose in the concentration range from 0.4 to 15 mM, with a detection limit of 0.4 mM. The sensor exhibited good selectivity and durability, with a long-term relative standard deviation of <5 %. Analysis of glucose-spiked human serum samples yielded recoveries between 96 and 101 %. [Figure not available: see fulltext.]

Subjects

AMPEROMETRIC sensors; HORSERADISH peroxidase; GLUCOSE oxidase; CARBON nanotubes; SERUM albumin; CHARGE exchange

Publication

Microchimica Acta, 2014, Vol 181, Issue 5/6, p535

ISSN

0026-3672

Publication type

Academic Journal

DOI

10.1007/s00604-014-1175-z