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- Title
Suppressive effect of ethanol on the expression of hepatic asialoglycoprotein receptors augmented by interleukin-1beta, interleukin-6, and tumor necrosis factor-alpha.
- Authors
Kato, Junji; Mogi, Yoshihiro; Kohgo, Yutaka; Takimoto, Rishu; Kobune, Masayoshi; Hisai, Hiroyuki; Nakamura, Tokiko; Takada, Kohichi; Niitsu, Yoshiro; Kato, J; Mogi, Y; Kohgo, Y; Takimoto, R; Kobune, M; Hisai, H; Nakamura, T; Takada, K; Niitsu, Y
- Abstract
Blood levels of inflammatory-related cytokines, including interleukin (IL)-1beta, IL-6, and tumor necrosis factor (TNF)-alpha, are elevated in patients with alcoholic liver diseases. We investigated the effects of these cytokines and ethanol on the expression of hepatic asialoglycoprotein receptors (AGPRs) in a human hepatoblastoma cell line, HepG2. An [125I]-asialo-orosomucoid binding assay showed significant increases in surface AGPR numbers in HepG2 cells by treatment with IL-1beta, IL-6, and TNF-alpha, to levels which were approximately 130% of the values in untreated control cells. However, the enhanced AGPR numbers induced by treatment with these cytokines were markedly suppressed, to 70%-80% of the number in the untreated cells, by treatment with ethanol. Immunological detection of AGPR with a specific antibody demonstrated that the modulation of surface AGPR numbers was correlated with the cellular expression levels of AGPR. These results suggest that, although IL-1beta, IL-6, and TNF-alpha stimulate the synthesis of hepatic AGPR, ethanol suppresses the expression of AGPR augmented by these cytokines. This leads to an increase in serum asialo-orosomucoid levels caused by the disordered catabolism mediated by AGPR in patients with alcoholic liver disease.
- Subjects
LIVER diseases; PHYSIOLOGICAL effects of alcohol; CYTOKINES; PHYSIOLOGY; DISEASE risk factors; CELL receptors; COMPARATIVE studies; ETHANOL; GLYCOPROTEINS; HEPATOCELLULAR carcinoma; INTERLEUKIN-1; INTERLEUKINS; LIVER tumors; RESEARCH methodology; MEDICAL cooperation; RESEARCH; TUMOR necrosis factors; EVALUATION research; CANCER cell culture
- Publication
Journal of Gastroenterology, 1998, Vol 33, Issue 6, p855
- ISSN
0944-1174
- Publication type
Academic Journal
- DOI
10.1007/s005350050187