Results
Title

Construction of a high efficiency copper adsorption bacterial system via peptide display and its application on copper dye polluted wastewater.

Authors

Maruthamuthu, Murali; Nadarajan, Saravanan; Ganesh, Irisappan; Ravikumar, Sambandam; Yun, Hyungdon; Yoo, Ik-keun; Hong, Soon

Abstract

For the construction of an efficient copper waste treatment system, a cell surface display strategy was employed. The copper adsorption ability of recombinant bacterial strains displaying three different copper binding peptides were evaluated in LB Luria-Bertani medium (LB), artificial wastewater, and copper phthalocyanine containing textile dye industry wastewater samples. Structural characteristics of the three peptides were also analyzed by similarity-based structure modeling. The best binding peptide was chosen for the construction of a dimeric peptide display and the adsorption ability of the monomeric and dimeric peptide displayed strains were compared. The dimeric peptide displayed strain showed superior copper adsorption in all three tested conditions (LB, artificial wastewater, and textile dye industry wastewater). When the strains were exposed to copper phthalocyanine dye polluted wastewater, the dimeric peptide display [543.27 µmol/g DCW dry cell weight (DCW)] showed higher adsorption of copper when compared with the monomeric strains (243.53 µmol/g DCW).

Subjects

COPPER binding proteins; ESCHERICHIA coli; PEPTIDE antibiotics; PHTHALOCYANINE synthesis; ELECTRIC batteries

Publication

Bioprocess & Biosystems Engineering, 2015, Vol 38, Issue 11, p2077

ISSN

1615-7591

Publication type

Academic Journal

DOI

10.1007/s00449-015-1447-y