Assessment of two immobilized lipases activity and stability to low temperatures in organic solvents under ultrasound-assisted irradiation.

Batistella, Luciane; Ustra, Mara; Richetti, Aline; Pergher, Sibele; Treichel, Helen; Oliveira, J.; Lerin, Lindomar; Oliveira, Débora

Both stability and catalytic activity of two commercial immobilized lipases were investigated in the presence of different organic solvents in ultrasound-assisted system. In a general way, for Novozym 435, the use of ethanol as solvent led to a loss of activity of 35% after 10 h of contact. The use of iso-octane conducted to a gradual increase in lipase activity in relation to the contact time, reaching a maximum value of relative activity of 126%. For Lipozyme RM IM, after 5 h of exposure, the enzyme presented no residual activity when ethanol was used as solvent. The solvents tert-butanol and iso-octane showed an enhancement of about 20 and 17% in the enzyme activity in 6 h of exposure, respectively. Novozym 435 and Lipozyme IM presented high stability to storage after treatment under ultrasound-assisted system using n-hexane and tert-butanol as solvents.

CATALYSIS research; ORGANIC solvents; LIPASES; TERT-Butyl halides; SOLVENTS

Bioprocess & Biosystems Engineering, 2012, Vol 35, Issue 3, p351

1615-7591

Academic Journal

10.1007/s00449-011-0573-4