Effects of increased skin blood flow on muscle oxygenation/deoxygenation: comparison of time-resolved and continuous-wave near-infrared spectroscopy signals.

Koga, Shunsaku; Poole, David; Kondo, Narihiko; Oue, Anna; Ohmae, Etsuko; Barstow, Thomas

Purpose: We quantified the contribution of skin blood flow (SkBF) to tissue oxygenation/deoxygenation of the flexor digitorum profundus muscle during cutaneous vasodilation. Methods: Time-resolved near-infrared spectroscopy (TRS-NIRS) was utilized to measure the potential influence of optical factors [mean optical pathlength (PL) and coefficients of absorption (μ) and reduced scattering ( $$\mu_{s}^{{\prime }}$$ )] on the NIRS-derived signals of eight male subjects. Results: The approximately threefold elevation of SkBF during 1 h whole-body heating (increased internal temperature ~0.9 °C) increased both μ and $$\mu_{s}^{{\prime }}$$ without changing PL. Assuming that the $$\mu_{s}^{{\prime }}$$ coefficient remained constant, i.e., as with continuous-wave (CW) NIRS, resulted in a significant increase in the apparent oxygenation [oxy(Hb Mb), from 113 ± 13 μM (mean ± SD) for control to 126 ± 13 for the increased SkBF condition, P < 0.01]: this was in marked contrast to the unchanged TRS-derived values. The deoxygenation [deoxy(Hb Mb)] also increased from control to elevated SkBF (CW-NIRS, from 39 ± 8 to 45 ± 7; TRS, from 38 ± 6 to 44 ± 7 μM; P < 0.01 for both), but less than that seen for oxy(Hb Mb) and not different between TRS- and CW-NIRS. Further, and in contrast to oxy(Hb Mb), temporal profiles of deoxy(Hb Mb) measured by the two NIRS methods were not different. Conclusions: These findings support use of either NIRS method to estimate local muscle fractional O extraction, but not oxygenation, when SkBF is increased at rest.

BLOOD flow; PHYSIOLOGICAL effects of oxygen; FLEXOR muscles; VASODILATION; NEAR infrared spectroscopy

European Journal of Applied Physiology, 2015, Vol 115, Issue 2, p335

1439-6319

Academic Journal

10.1007/s00421-014-3019-2