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- Title
Effect of different resistance-training regimens on the WNT-signaling pathway.
- Authors
Leal, Marcelo; Lamas, Leonardo; Aoki, Marcelo; Ugrinowitsch, Carlos; Ramos, Marcela; Tricoli, Valmor; Moriscot, Anselmo; Leal, Marcelo Larciprete; Aoki, Marcelo Saldanha; Ramos, Marcela Sorelli Carneiro; Moriscot, Anselmo Sigari
- Abstract
The purpose of the present study was to evaluate the effects of 8 weeks of strength and power training on the expression of genes related to the canonical WNT pathway and β-catenin protein levels in physically active men. Twenty-five subjects (27.4 ± 4.6 years) were balanced based on their relative maximum strength in the squat exercise (squat 1RM/body mass) and randomly assigned to strength training (ST) (n = 10), power training (PT) (n = 10), and control (C) (n = 5) groups. The ST and the PT groups performed high and low intensity squats, respectively, thrice a week, for 8 weeks. Muscle biopsies from the vastus lateralis muscle were collected before and after the training period. Relative strength and power increased similarly in both ST and PT groups (P < 0.001). Fiber cross-sectional area also increased similarly in both ST and PT groups. Gene expression and β-catenin protein expression levels were assessed by real-time PCR and Western blot. Certain genes were up-regulated in the ST group (WNT1: 6.4-fold, P < 0.0001; SFRP1: 3.3-fold, P < 0.0001 and LEF1: 7.3-fold, P < 0.0001) and also in the PT group (WNT1: 24.9-fold, P < 0.0001; SFRP1: 2.7-fold, P < 0.0001; LEF1: 34.1-fold, P < 0.0001 and Cyclin D1: 7.7-fold, P < 0.001). In addition, the expression of key WNT pathway genes was substantially more responsive to PT than to ST (WNT1: P < 0.0001; LEF1: P < 0.0001 and Cyclin D1: P < 0.001). Finally, the total β-catenin protein content increased only in the PT group (P < 0.05). Our data indicate that a PT regimen triggers greater responses in key elements of the WNT pathway.
- Subjects
GENE expression; SKELETAL muscle; VASTUS lateralis; WNT genes; POLYMERASE chain reaction; WESTERN immunoblotting; HYPERTROPHY; PROTEIN synthesis; PROTEIN metabolism; SKELETAL muscle physiology; ALGORITHMS; CELLULAR signal transduction; COMPARATIVE studies; CYTOSKELETAL proteins; RESEARCH methodology; MEDICAL cooperation; MUSCLE strength; PHYSICAL fitness; PROTEINS; RESEARCH; EVALUATION research; RANDOMIZED controlled trials; SEDENTARY lifestyles; GENE expression profiling; RESISTANCE training
- Publication
European Journal of Applied Physiology, 2011, Vol 111, Issue 10, p2535
- ISSN
1439-6319
- Publication type
Academic Journal
- DOI
10.1007/s00421-011-1874-7