Results
Title

Fetal growth at term and placental oxidative stress in a tissue micro-array model: a histological and immunohistochemistry study.

Authors

Xodo, Serena; Celante, Lisa; Liviero, Stefania; Orsaria, Maria; Mariuzzi, Laura; De Luca, Matteo; Damante, Giuseppe; Driul, Lorenza; Cagnacci, Angelo; Ferino, Annalisa; Di Giorgio, Eros; Xodo, Luigi; Londero, Ambrogio Pietro

Abstract

This study examines 8-hydroxyguanine (8-oxo-Gua) staining in placental tissue samples based on fetal size at birth as well as its relationships with placental histology and other pregnancy variables. This prospective cohort study included women > 18 years with a singleton pregnancy, a live fetus, fluency in Italian, and delivery at term. A total of 165 pregnancies were included in the study. The nuclear syncytiotrophoblast 8-oxo-Gua staining score in LGA was substantially greater than in late FGR (p < 0.05), although the cytoplasm score was lower in SGA and LGA than in AGA (p < 0.05). Furthermore, a sex-specific pattern of 8-oxo-Gua staining was discovered in single-term placentas, with more oxidative damage found in the nuclei of syncytiotrophoblast cells and stromal and endothelial cells in AGA males compared to AGA females (p < 0.05). Second, the histological pattern of late FGR placentae differed by gender. Finally, a significant correlation (p < 0.05) was found between high-intensity 8-oxo-Gua staining in the cytoplasm of syncytiotrophoblast cells and thrombi in the chorionic plate or villi in males. On the other hand, female fetuses demonstrated a significant connection (p < 0.05) between high-intensity 8-oxo-Gua staining in endothelial and stromal cells and high birthweight MoM values. Our findings indicated a significant variation in the oxidative stress pattern between male and female placentae, implying that fetal growth is regulated differently in the two sexes.

Subjects

FETAL development; OXIDATIVE stress; CHORIONIC villi; FETUS; PLACENTA; BIRTH size

Publication

Histochemistry & Cell Biology, 2023, Vol 160, Issue 4, p293

ISSN

0948-6143

Publication type

Academic Journal

DOI

10.1007/s00418-023-02212-6