Combined Proteomics and Physiological Analyses Reveal Drought and Recovery Response Mechanisms in Banana Leaves.

Lau, Su-Ee; Pua, Teen-Lee; Saidi, Noor Baity; Ong-Abdullah, Janna; Lamasudin, Dhilia Udie; Tan, Boon Chin

Drought is the most prominent limiting factor to crop productivity. Banana, one of the most important food crops globally, is highly susceptible to drought. However, how banana plants respond to drought stress and post-drought recovery remains unclear. Therefore, this study determined the morphological and protein responses of banana plants (Musa acuminata cultivar Berangan) affected by drought stress, followed by recovery. The results showed that drought significantly reduced the leaf area, plant height, fresh weight, stem circumference, leaf relative water content, chlorophyll contents, and root length of the bananas. In contrast, relative electrolyte leakage, proline, malondialdehyde (MDA) and hydrogen peroxide contents, and the activities of antioxidant enzymes, including catalase (CAT), ascorbate peroxidase (APX), glutathione reductase (GR), peroxidase, and superoxide dismutase, were induced in the drought-treated banana leaves. However, after recovering from drought stress, the relative water content, MDA, hydrogen peroxide, and antioxidant enzyme activities, including CAT, APX, and GR, were comparable with well-watered plants. To determine the protein responses of bananas toward drought stress, the well-watered, drought-stressed, and recovered banana leaves were sampled for tandem mass tags-based quantitative proteomics analysis. Of the 1018 differentially abundant proteins, 274 were significantly changed. The identified proteins differing between the treatments were mainly related to carbohydrate, energy and amino acid metabolisms, genetic information processing, and secondary metabolite biosynthesis. Our data may assist in developing a complete proteome dataset which could be valuable for developing drought-tolerant bananas.

BANANAS; DROUGHTS; PROTEOMICS; FOOD crops; AMINO acid metabolism; GLUTATHIONE reductase; SUPEROXIDE dismutase

Journal of Plant Growth Regulation, 2023, Vol 42, Issue 12, p7624

0721-7595

Academic Journal

10.1007/s00344-023-11039-3