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- Title
Limnobacter olei sp. nov., a Novel Diesel-Degrading Bacterium Isolated from Oil-Contaminated Soil.
- Authors
Liu, Yongchuang; Xu, Siqiong; Li, Jialiang; Ouyang, Yujie; Gao, Shuai; Yang, Pan; Chu, Cuiwei; He, Jian; Yang, Tongwen; Ma, Keshi; Li, Lili; Wang, Chao
- Abstract
A bacterial strain P1T, capable of degrading diesel and converting thiosulfate to sulfate was isolated from an oil-contaminated soil sample. The cells were Gram-stain-negative, slightly curved rods and motile with a single polar flagellum. Growth of the strain was observed at 4–45 °C (optimum at 28 °C), at pH 4.0–12.0 (optimum at pH 10.0) and with 0–15.0% (w/v) NaCl (optimum at 2.0%). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain P1T was closely related to the members of the genus Limnobacter, with the highest sequence similarity to Limnobacter thiooxidans DSM 13612 T (99.8%), followed by Limnobacter alexandrii LZ-4 T (99.4%), Limnobacter parvus YS8-69 T (98.8%), Limnobacter litoralis KP1-19 T (97.6%), and Limnobacter humi UCM-39 T (97.5%). The draft genome sequence of strain P1T was 3.40 Mb long, with a DNA G + C content of 52.4%. The average nucleotide identity and digital DNA-DNA hybridization values between strain P1T and the closely related type strains were in the range of 71.8–85.1% and 18.1–28.7%, respectively. The predominant cellular fatty acids of strain P1T included C16: 0, summed feature 3 (C16: 1ɷ7c and/or C16: 1ɷ6c), summed feature 8 (C18: 1ɷ7c and/or C18: 1ɷ6c), and summed feature 7 (C19: 1ɷ6c and/or C19: 1ɷ7c and/or C19: 1 cyclo). In addition, the main polar lipid was composed of diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylglycerol. Q-8 was the sole respiratory quinone. Based on the polyphasic characterization, strain P1T (= KCTC 72814 T = CCTCC AB 2019403 T) represents a novel species of the genus Limnobacter, for which the name Limnobacter olei sp. nov. is proposed.
- Subjects
NUCLEIC acid hybridization; SOIL sampling; PHOSPHATIDYLGLYCEROL; FATTY acids; FLAGELLA (Microbiology)
- Publication
Current Microbiology, 2025, Vol 82, Issue 2, p1
- ISSN
0343-8651
- Publication type
Academic Journal
- DOI
10.1007/s00284-024-04053-5