We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Differentiation of endothelial cells from human umbilical cord blood AC133-CD14 cells.
- Authors
Kim, Shin-Young; Park, So-Yeon; Kim, Jin; Kim, Jin-Woo; Kim, Moon; Yang, Jae; Kim, Joo; Choi, Kyu-Hong; Kim, Seung; Ryu, Hyun-Mee; Kim, Jin Mi; Kim, Moon Young; Yang, Jae Hyug; Kim, Joo Oh; Kim, Seung Bo
- Abstract
Endothelial progenitor cells (EPCs) participate in neovascularization and are consistent with postnatal vasculogenesis. In vitro, they differentiate into endothelial cells (ECs). Prior reports have suggested that circulating human AC133 cells have the capacity to differentiate into ECs as progenitor cells. However, recent studies have demonstrated that circulating CD34−CD14 cells also have EPC-like properties in vitro and in vivo. We tested whether AC133−CD14 cells from human umbilical cord blood (HUCB) have the potential to differentiate into ECs. The AC133−CD14 cells were isolated from HUCB by magnetic bead selection and cultured on fibronectin-coated six-well trays in M199 medium supplemented with fetal bovine serum (FBS), vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and insulin growth factor (IGF-1). The AC133−CD14 cells adhered slightly within 1 day of culture and subsequently underwent a distinct process of morphological transformation to spindle-shaped cells that sprouted from the edge of the cell clusters. After 14 days, the cells formed cord- and tubular-like structures. The AC133−CD14 cells showed a strong increase in the endothelial marker P1H12 over time, whereas CD14 decreased, and CD45 did not change, respectively. In addition, the cells expressed endothelial markers von Willebrand’s factor (vWF), platelet/endothelial cell adhesion molecule-1 (PECAM-1), vascular endothelial growth factor receptor-1 (VEGFR-1)/Flt-1, VEGFR-2/Flk-1, eNOS, and VE-cadherin, but did not express Tie-2 after 7 days of culture. The present data indicate that AC133−CD14 cells from HUCB are able to develop endothelial phenotype with expression of endothelial-specific surface markers and even form cord- and tubular-like structures in vitro as progenitor cells.
- Subjects
MOLECULAR cell differentiation; NEOVASCULARIZATION; VASCULAR endothelium; VASCULAR endothelial growth factors; CD antigens; BLOOD-vessel development; CORD blood
- Publication
Annals of Hematology, 2005, Vol 84, Issue 7, p417
- ISSN
0939-5555
- Publication type
Academic Journal
- DOI
10.1007/s00277-004-0988-y