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- Title
The antibacterial activity of LI-F type peptide against methicillin-resistant <italic>Staphylococcus aureus</italic> (MRSA) in vitro and inhibition of infections in murine scalded epidermis.
- Authors
Han, Jinzhi; Ma, Zhi; Gao, Peng; Lu, Zhaoxin; Liu, Hongxia; Gao, Ling; Lu, Wenjun; Ju, Xiangyu; Lv, Fengxia; Zhao, Haizhen; Bie, Xiaomei
- Abstract
LI-F type peptides are a family of cyclic lipodepsipeptide antibiotics isolated from Paenibacillus polymyxa and display potent activities against positive bacteria including methicillin-resistant S. aureus (MRSA). In this study, we investigated the mechanism of action of LI-F type peptide AMP-jsa9 against a MRSA (S. aureus CICC10790), which is resistant to ciprofloxacin, gentamicin, kanamycin, chloramphenicol, methicillin, and tetracycline. It was found that AMP-jsa9 mainly targets the cell membrane of MRSA and is able to inhibit biofilm formation through killing planktonic bacteria cells. Moreover, AMP-jsa9 can bind to DNA in vitro, which represents another pathway for the action on MRSA. Furthermore, in vivo treatment of scalded mice with AMP-jsa9 resulted in inhibiting MRSA infections and healing of the scalded wound. In addition, it was demonstrated that AMP-jsa9 can effectively inhibit MRSA infections in scalded murine epidermis and that inflammatory cytokines including IL-8, IL-6, tumor necrosis factor alpha (TNF-α), and monocyte chemotactic factor-1 (MCP-1) were reduced; moreover, both protein and gene expression levels of vascular endothelial growth factor (VEGF) and endothelial nitric oxide synthase (e-NOS) were enhanced, which promote neovascularization and proliferation of new granulation tissue.
- Subjects
DEPSIPEPTIDES; PAENIBACILLUS; METHICILLIN-resistant staphylococcus aureus; CIPROFLOXACIN; NEOVASCULARIZATION; NITRIC oxide synthesis
- Publication
Applied Microbiology & Biotechnology, 2018, Vol 102, Issue 5, p2301
- ISSN
0175-7598
- Publication type
Academic Journal
- DOI
10.1007/s00253-017-8669-7