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- Title
Molecular engineering of industrial enzymes: recent advances and future prospects.
- Authors
Yang, Haiquan; Li, Jianghua; Shin, Hyun-dong; Du, Guocheng; Liu, Long; Chen, Jian
- Abstract
Many enzymes are efficiently produced by microbes. However, the use of natural enzymes as biocatalysts has limitations such as low catalytic efficiency, low activity, and low stability, especially under industrial conditions. Many protein engineering technologies have been developed to modify natural enzymes and eliminate these limitations. Commonly used protein engineering strategies include directed evolution, site-directed mutagenesis, truncation, and terminal fusion. This review summarizes recent advances in the molecular engineering of industrial enzymes and discusses future prospects in this field. We expect this review to increase interest in and advance the molecular engineering of industrial enzymes.
- Subjects
EVOLUTIONARY theories; SITE-specific mutagenesis; PROTEIN engineering; ENZYME kinetics; ENZYMES; ENZYME stability
- Publication
Applied Microbiology & Biotechnology, 2014, Vol 98, Issue 1, p23
- ISSN
0175-7598
- Publication type
Academic Journal
- DOI
10.1007/s00253-013-5370-3