EBSCO Logo
Connecting you to content on EBSCOhost
Results
Title

Characterization of thermostable FMN-dependent NADH azoreductase from the moderate thermophile Geobacillus stearothermophilus.

Authors

Matsumoto, Ken’ichiro; Mukai, Yuichi; Ogata, Daiki; Shozui, Fumi; Nduko, John; Taguchi, Seiichi; Ooi, Toshihiko

Abstract

The gene encoding an FMN-dependent NADH azoreductase, AzrG, from thermophilic Geobacillus stearothermophilus was cloned and functionally expressed in recombinant Escherichia coli. Purified recombinant AzrG is a homodimer of 23 kDa and bore FMN as a flavin cofactor. The optimal temperature of AzrG was 85 °C for the degradation of Methyl Red (MR). AzrG remained active for 1 h at 65 °C and for 1 month at 30 °C, demonstrating both superior thermostability and long-term stability of the enzyme. AzrG efficiently decolorized MR, Ethyl Red at 30 °C. Furthermore, the enzyme exhibited a wide-range of degrading activity towards several tenacious azo dyes, such as Acid Red 88, Orange I, and Congo Red. These results suggested the sustainable utilization of G. stearothermophilus as an azo-degrading strain for AzrG carrying whole-cell wastewater treatments for azo pollutants under high temperature conditions.

Subjects

FLAVOPROTEINS; OXIDOREDUCTASES; ESCHERICHIA coli; GRAM-negative bacteria; WASTEWATER treatment; SEWAGE purification; CIRCULAR dichroism; AZO dyes; POLLUTANTS

Publication

Applied Microbiology & Biotechnology, 2010, Vol 86, Issue 5, p1431

ISSN

0175-7598

Publication type

Academic Journal

DOI

10.1007/s00253-009-2351-7

EBSCO Connect | Privacy policy | Terms of use | Copyright | Manage my cookies
Journals | Subjects | Sitemap
© 2025 EBSCO Industries, Inc. All rights reserved