SERS assessment of the cancer-specific methylation pattern of genomic DNA: towards the detection of acute myeloid leukemia in patients undergoing hematopoietic stem cell transplantation.

Moisoiu, Vlad; Stefancu, Andrei; Iancu, Stefania D.; Moisoiu, Tudor; Loga, Luminita; Dican, Lucia; Alecsa, Cristian D.; Boros, Imre; Jurj, Anca; Dima, Delia; Bagacean, Cristina; Tetean, Romulus; Burzo, Emil; Tomuleasa, Ciprian; Elec, Florin; Leopold, Nicolae

In this label-free surface-enhanced Raman scattering (SERS) study of genomic DNA, we demonstrate that the cancer-specific DNA methylation pattern translates into specific spectral differences. Thus, DNA extracted from an acute myeloid leukemia (AML) cell line presented a decreased intensity of the 1005 cm−1 band of 5-methylcytosine compared to normal DNA, in line with the well-described hypomethylation of cancer DNA. The unique methylation pattern of cancer DNA also influences the DNA adsorption geometry, resulting in higher adenine SERS intensities for cancer DNA. The possibility of detecting cancer DNA based on its SERS spectrum was validated on peripheral blood genomic DNA samples from n = 17 AML patients and n = 17 control samples, yielding an overall classification of 82% based on the 1005 cm−1 band of 5-methylcytosine. By demonstrating the potential of SERS in assessing the methylation status in the case of real-life DNA samples, the study paves the way for novel methods of diagnosing cancer.

HEMATOPOIETIC stem cell transplantation; ACUTE myeloid leukemia; HEMATOPOIETIC growth factors; DNA methylation; CIRCULATING tumor DNA; DNA; METHYLATION; RAMAN scattering

Analytical & Bioanalytical Chemistry, 2019, Vol 411, Issue 29, p7907

1618-2642

Academic Journal

10.1007/s00216-019-02213-2